For over 35 years, biological scientists have come to rely on the research protocols and

methodologies in the critically acclaimed Methods in Molecular Biology series. The series was

the first to introduce the step-by-step protocols approach that has become the standard in all

biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-

step fashion, opening with an introductory overview, a list of the materials and reagents

needed to complete the experiment, and followed by a detailed procedure that is supported

with a helpful notes section offering tips and tricks of the trade as well as troubleshooting

advice. These hallmark features were introduced by series editor Dr. John Walker and

constitute the key ingredient in each and every volume of the Methods in Molecular Biology

series. Tested and trusted, comprehensive and reliable, all protocols from the series are

indexed in Pub Med.